Thermo Fisher Scientific NFkB p50 Polyclonal Antibody
NFkB p50 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, ELISA, Flow cytometry, Immunoprecipitation 등에 사용 가능. Human 시료 반응성, 고농도(80 mg/mL) 액상 형태, -20°C 보관 권장.
- 카탈로그번호
- 100-4164x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific NFkB p50 Polyclonal Antibody
Thermo Fisher Scientific NFkB p50 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 0.5–1.0 µL |
| ELISA | 1:5,000–1:25,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 80 mg/mL |
| Storage Buffer | Whole serum |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Product stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
- Anti-Human NFkB p50 (NFKB1) may react non-specifically with other proteins.
- Control peptide (code #100-4164p) will compete only with the specific reaction of antiserum with Human NFkB p50 (NFKB1).
Target Information
NF-kB is a nuclear transcription factor activated by various extra- and intracellular stimuli such as cytokines, UV radiation, stress, injury, and bacterial or viral products. It regulates cellular events including growth, differentiation, proliferation, apoptosis, and inflammation.
NFKB1 (p50), a 50 kDa functional subunit of NF-kB, belongs to the Rel protein family. It is synthesized as a p105 precursor containing an N-terminal conserved RHD region with nuclear localization, DNA-binding, and dimerization domains.
NFKB1 (p50) forms homodimers or heterodimers with p65 to form functional NF-kB. It directs nuclear translocation and DNA binding of NF-kB.
Pathological roles have been suggested in AIDS, hematogenic cancer metastasis, and rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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