Thermo Fisher Scientific Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), NovaFluor Yellow 755, eBioscience

Thermo Fisher Scientific Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), NovaFluor Yellow 755

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.8 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• Biotin
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• PE
• Custom conjugation available

Product Specific Information

The eBio13G3-19D monoclonal antibody reacts with mouse Ly108, the mouse homologue of human SLAMF6. Ly108 (also known as NTB-A) is expressed on NK, T, and B cells. Engagement enhances NK cell activity and IFN-γ expression, and is linked to X-linked lymphoproliferative disease mechanisms.

Staining shows no strain differences between C57BL/6 and BALB/c mice. Other strains not tested.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes and thymocytes at ≤0.8 µg/test (100 µL final volume). Optimal titration recommended.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability testing.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining. Add 5 µL per sample or per 100 µL of cell suspension (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, enabling engineered fluorescent signatures optimized for minimal spillover and spread.

Excitation: 552 nm
Emission: 690 nm
Laser: 561 nm (Yellow)

Internal testing indicates non-specific staining of B cells in SJL mice with NovaFluor Yellow 755; not observed in BALB/c or C57BL/6 strains.

Target Information

Ly108 is the mouse homologue of human SLAMF6, expressed on NK, B, and T cells. Activation enhances NK cell activity and IFN-γ expression.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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