Thermo Fisher Scientific Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), NovaFluor Yellow 700, eBioscience

Applications

• Flow Cytometry (Flow): Tested at ≤ 0.8 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• Custom conjugation available

Product Specific Information

The eBio13G3-19D monoclonal antibody recognizes mouse Ly108, the homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6). Ly108, also known as NTB-A, is expressed on NK, T, and B cells. Engagement of Ly108 enhances NK cell activity and IFN-γ expression and is implicated in immune regulation.

No strain differences were observed between C57BL/6 and BALB/c mice. Other strains have not been tested.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes and thymocytes at ≤ 0.8 µg/test (100 µL final volume). Cell density: 10⁵–10⁸ cells/test. Optimize titration for best performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell Stains for viability analysis.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining. Add 5 µL per sample before combining with cells.

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures designed to minimize spillover and spectral overlap.

• Excitation: 552 nm
• Emission: 700 nm
• Recommended Laser: 561 nm (Yellow)

Note:
Internal testing shows that NovaFluor Yellow 700 may non-specifically stain B cells in SJL mice, but not in BALB/c or C57BL/6 strains.

Target Information

Ly108 (mouse SLAMF6 homologue) is expressed on NK, T, and B cells. Activation enhances NK cell activity and IFN-γ expression, contributing to immune regulation.

For Research Use Only. Not for diagnostic or resale purposes.

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