Thermo Fisher Scientific Hydroxyl-Histone H2A (Tyr39) Recombinant Rabbit Monoclonal Antibody (SR4-17)
Western blot과 IHC(P)에서 검증된 Hydroxyl-Histone H2A (Tyr39) 항체로, 사람·마우스·랫트 반응성. 재조합 토끼 단클론 항체로 높은 특이성과 재현성을 제공. Protein A 정제 및 TBS 기반 완충액에 보존. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SR4-17 |
| Immunogen | Recombinant protein within Human Histone H2A (aa 1–83) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809298 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed in these systems. Individual clones are screened to select the best candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Two molecules each of H2A, H2B, H3, and H4 form an octamer, around which approximately 146 bp of DNA is wrapped to form nucleosomes.
The linker histone H1 interacts with linker DNA between nucleosomes and functions in chromatin compaction. The gene encoding this histone is intronless and lacks a polyA tail, instead containing a palindromic termination element. It is located in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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