
Thermo Fisher Scientific ELK3 Monoclonal Antibody (OTI1H3), TrueMAB
ELK3 단백질을 인식하는 Mouse 모노클로날 항체로 Western blot, IHC, ICC/IF, Flow cytometry에 사용 가능. Human 시료 반응. Lyophilized 형태로 제공되며, PBS buffer에 trehalose 포함. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H3 |
| Immunogen | Full length human recombinant protein of human ELK3 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882) before use.
Target Information
The ELK3 gene encodes a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily.
Proteins in this subfamily regulate transcription via interaction with serum response factor and binding to serum response elements.
ELK3 is activated by signal-induced phosphorylation. Studies in rodents indicate that it functions as a transcriptional inhibitor in the absence of Ras but activates transcription when Ras is present.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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