Thermo Fisher Scientific ELK3 Monoclonal Antibody (OTI2C10), TrueMAB
Human ELK3 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC/IF, 유세포분석에 사용 가능. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2C10 |
| Immunogen | Full length human recombinant protein of human ELK3 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The ELK3 protein is a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily.
These proteins regulate transcription by binding to serum response elements when recruited by serum response factor (SRF).
ELK3 is activated by signal-induced phosphorylation and functions as a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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