Thermo Fisher Scientific CD39 Monoclonal Antibody (eBioA1 (A1)), NovaFluor Yellow 660, eBioscience

Thermo Fisher Scientific CD39 Monoclonal Antibody (eBioA1 (A1)), NovaFluor Yellow 660

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant UV 805
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBioA1 monoclonal antibody reacts with human CD39 (ENTPD1, NTPDase).
CD39 is an integral membrane protein with two transmembrane domains and exists as a homotetramer. It removes extracellular ATP by converting it to ADP or AMP and works together with CD73 to hydrolyze ATP. Expression is found on subsets of T cells, B cells, dendritic cells, and weakly on monocytes and granulocytes. CD39 and CD73 are expressed on regulatory T cells (Treg), aiding their migration into inflamed areas with high ATP levels.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use: 5 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability testing.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates are based on Phiton™ technology, using nucleic acid dye structures engineered for minimal spillover and optimized spectral performance.

Excitation: 552 nm
Emission: 663 nm
Laser: 561 nm (Yellow)

Target Information

CD39 is a 70–100 kDa cell surface antigen expressed on peripheral blood B cells, monocytes, T cell clones, and weakly on granulocytes. It has intrinsic ecto-ATPase activity and is induced on T cells and increased on B cells as a late activation antigen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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