Thermo Fisher Scientific CD39 Monoclonal Antibody (eBioA1 (A1)), NovaFluor Yellow 755, eBioscience

Thermo Fisher Scientific CD39 Monoclonal Antibody (eBioA1 (A1)), NovaFluor Yellow 755

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant UV 805
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBioA1 monoclonal antibody reacts with human CD39 (ENTPD1 or NTPDase), an integral membrane protein functioning as an ectoenzyme that converts extracellular ATP to ADP or AMP. CD39 is expressed on activated lymphocytes, subsets of T and B cells, dendritic cells, and weakly on monocytes and granulocytes.

CD39 and CD73 are co-expressed on regulatory T cells (Treg), facilitating their migration into inflamed areas with high ATP levels. Expression of CD39 on Foxp3+CD4+ cells ranges from 25–45%.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-titrated and validated on normal human peripheral blood cells. Use 5 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Compatibility Note:
NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.

CellBlox Plus Blocking Buffer:
This improved buffer (Cat. No. C001T06F01) enhances staining performance with NovaFluor conjugates. Add 5 µL per sample before mixing with cells. For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor Technology:
Based on Phiton™ technology utilizing engineered nucleic acid dye structures, providing optimized fluorescent signatures with reduced spillover and spread.

Excitation: 552 nm
Emission: 690 nm
Laser: 561 nm (Yellow)

Target Information

CD39 is a 70–100 kDa cell surface molecule expressed on peripheral blood B cells, monocytes, T cell clones, and weakly on granulocytes. It exhibits ecto-ATPase activity and is upregulated on activated T and B cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

제품 이미지