Thermo Fisher Scientific IL-27 p28 Recombinant Rabbit Monoclonal Antibody (7H7L10)

Thermo Fisher Scientific IL-27 p28 Recombinant Rabbit Monoclonal Antibody (7H7L10)

Applications and Tested Dilution

• Western Blot (WB): 0.1–1 µg/mL

Product Specifications

Product Specific Information

• Intact IgG appears on a non-reducing gel as a ~150 kDa band.
  Upon reduction, it generates a ~25 kDa light chain and a ~50 kDa heavy chain.
• Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems.
  These systems clone antibody DNA sequences from immunoreactive rabbits and select high-performance clones for production.
• Advantages include:
  • Enhanced specificity and sensitivity
  • Lot-to-lot consistency
  • Animal origin-free formulations
  • Broader immunoreactivity due to the rabbit immune repertoire

Target Information

IL-27 is a member of the IL-12 family, composed of the p40-related protein EBI3 non-covalently linked to the IL-12p35-related protein p28 (IL-30).
It is produced by activated antigen-presenting cells and mature dendritic cells.
IL-27 acts on NK cells and naive CD4+ T cells, and may also influence mast cells and monocytes.

Binding of IL-27 to WSX-1/gp130 activates JAK1, STAT1, and STAT3 pathways.
Deficiency in WSX-1/TCCR leads to impaired Th1 responses and higher susceptibility to infection, indicating IL-27’s role in Th1 initiation.
IL-27 induces Th1-specific transcription factor T-bet and suppresses Th2-specific GATA-3, contributing to Th1 differentiation.
Recent studies show IL-27 has antitumor activity, enhancing CTL function, inhibiting tumor growth, and inducing regression in neuroblastoma models.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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