Thermo Fisher Scientific PRAS40 Recombinant Rabbit Monoclonal Antibody (4H14L8)

Applications

Western Blot (WB)

• Tested Dilution: 1:100–1:1,000
• View 1 publication

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500
• View 1 publication

Product Specifications

Product Specific Information

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire

Target Information

The Akt signaling pathway contributes to the regulation of apoptosis after various cell death signals.
AKT1S1 (also known as PRAS40) is a proline-rich substrate of the kinase AKT1 and plays a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia.
AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and acts as a negative regulator of autophagy.
Treatment with insulin-like growth factor-1 (IGF1) can induce phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.