Thermo Fisher Scientific iNOS Monoclonal Antibody (CXNFT), Brilliant Violet 605, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 786
• eFluor 450
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This CXNFT monoclonal antibody reacts to mouse NOS2 (inducible NOS, iNOS). Nitric oxide synthase enzymes catalyze the formation of nitric oxide from L-arginine through an NADPH- and oxygen-dependent mechanism.
There are three isoforms of NOS: NOS1 (nNOS), NOS2 (iNOS), and NOS3 (eNOS). NOS2 is induced by bacterial endotoxins and inflammatory cytokines such as IFN-γ and TNF-α, and is expressed by myeloid-derived suppressor cells and M1 macrophages.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested using mouse thioglycolate-elicited peritoneal exudate cells with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
Recommended use ≤0.5 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Titrate for optimal performance.

Fluorochrome Information:
Brilliant Violet™ 605 (BV605) emits at 605 nm and is intended for violet (405 nm) laser-equipped cytometers.

Staining Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54).
Samples can be stored up to 3 days at 4°C in the dark.
Not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 605 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

iNOS (Inducible Nitric Oxide Synthase) produces nitric oxide (NO), a gaseous signaling molecule involved in vasorelaxation, neurotransmission, and immune response.
It is expressed in various cell types including macrophages, hepatocytes, and smooth muscle cells.
Cytokines such as IFN-γ, TNF, IL-1, IL-2, and LPS induce iNOS expression.
Three related iNOS pseudogenes are located on chromosome 17.
Dysregulation of iNOS is associated with diseases such as achalasia and impotence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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