Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), Brilliant Violet 605, eBioscience
마우스 IgM μ 중쇄를 인식하는 단클론 항체로, Flow Cytometry에 최적화되어 있습니다. Brilliant Violet 605 형광체로 결합되어 405 nm 레이저 사용 장비에 적합합니다. 고순도 정제 및 안정적 신호 제공, 4°C 보관 및 빛 차단 필요.
- 카탈로그번호
- 406-5790-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), Brilliant Violet 605, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 1.0 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Violet™ 605, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | II/41 |
| Conjugate | Brilliant Violet™ 605 |
| Excitation/Emission Max | 406/603 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_3074083 |
Additional Formats Available
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 711
- eFluor 450
- eFluor 570
- eFluor 660
- FITC
- NovaFluor Red 710
- NovaFluor Yellow 610
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 780
- Request custom conjugation
Product Specific Information
The II/41 monoclonal antibody reacts with the μ heavy chain of mouse IgM and does not cross-react with IgD, IgG, or IgA. IgM is expressed intracellularly during early B cell development and on the surface of mature B cells in bone marrow and peripheral tissues. This fluorochrome-conjugated antibody can be used as a detection secondary for mouse IgM in flow cytometry.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on mouse bone marrow cells
- Recommended Usage: ≤1.0 µg/test (in 100 µL final volume)
Brilliant Violet™ 605 (BV605) emits at 605 nm and is optimized for cytometers with a 405 nm laser. Ensure instrument compatibility before use.
When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.
Light Sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark.
Note: BV605 is not compatible with methanol-based fixation.
Excitation: 407 nm
Emission: 605 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
Mouse IgM is an immunoglobulin M antibody produced by B cells in response to antigens. It is the first antibody generated during an immune response and the largest immunoglobulin in serum. Composed of five heavy and five light chains, IgM is key in complement activation and antigen agglutination. It plays a crucial role in innate immunity and is widely used in research applications such as ELISA, Western blotting, and immunohistochemistry.
For Research Use Only. Not for use in diagnostic procedures.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.