Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), Brilliant Violet 605, eBioscience
마우스 NK1.1 항원을 인식하는 PK136 단클론 항체로, Brilliant Violet 605 형광체가 결합되어 있습니다. 유세포 분석에 최적화되어 있으며, C57BL/6 등 NK1.1 발현 마우스에서 NK 세포 검출에 사용됩니다. 405 nm 레이저 기반 장비에 적합하며, 빛과 메탄올 고정에 민감합니다.
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Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), Brilliant Violet 605, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 1.0 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PK136 |
| Conjugate | Brilliant Violet™ 605 |
| Excitation/Emission Max | 406 / 603 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2937182 |
Available Formats
- Unconjugated
- Alexa Fluor 561
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 661 / 805
- Brilliant Violet 421 / 480 / 650 / 711 / 786
- eFluor 450
- FITC
- Functional Grade
- PE / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation available
Product Specific Information
The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer (NK) cells and a subset of T cells in NK1.1 mouse strains such as C57BL and NZB. Strains like BALB/c, SJL, AKR, CBA, C3H, and A do not express NK1.1 antigen.
For these strains, use monoclonal antibody DX5 (Product #14-5971) for NK cell detection.
Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 shows coexpression of both markers by most cells, with small populations of DX5⁺PK136⁻ and DX5⁻PK136⁺ cells.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume).
Optimal titration recommended for best assay performance.
Brilliant Violet™ 605 (BV605) is a tandem dye emitting at 605 nm, designed for cytometers with a 405 nm violet laser. Ensure instrument compatibility.
When using multiple Brilliant Violet™, Brilliant Ultra Violet™, or polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to minimize non-specific interactions.
Light Sensitivity: Protect vial and stained samples from light to avoid photo-induced oxidation.
Fixation: Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54).
Compatibility: BV605 is not compatible with methanol-based fixation.
- Excitation: 407 nm
- Emission: 605 nm
- Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
NK1.1 (KLRB1, NKR-P1A, CD161) is a receptor expressed by Natural Killer (NK) cells, which mediate cytotoxicity and cytokine secretion.
The KLRB1 protein belongs to the C-type lectin superfamily, containing extracellular lectin motifs, a transmembrane domain, and a cytoplasmic domain.
It is classified as a type II membrane protein with an external C terminus.
The receptor recognizes Lectin Like Transcript-1 (LLT1) as its functional ligand.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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