Thermo Fisher Scientific VEGF Receptor 2 Polyclonal Antibody
VEGF Receptor 2를 인식하는 염소 유래 폴리클로날 항체로, WB, IHC, Flow, Neutralization에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 비보존제 PBS 용액 형태로 제공. 마우스 VEGFR2 연구용에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| Immunohistochemistry (IHC) | 5–15 µg/mL |
| Flow Cytometry (Flow) | 3–10 µg/mL |
| Neutralization (Neu) | 0.1–0.3 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant extracellular domain of mouse VEGF receptor 2, expressed in mouse NSO cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_561911 |
Product Specific Information
Neutralization: Anti-Mouse VEGF Receptor 2 has the ability to neutralize the biological activity of recombinant mouse VEGF Receptor 2 on HUVE (human umbilical vein endothelial) cells in the presence of recombinant mouse VEGF Receptor 2/Fc (50 ng/mL) and recombinant mouse VEGF (5 ng/mL).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase that binds vascular endothelial growth factor with high affinity in vitro and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of VEGF, known as kinase insert domain receptor. The signaling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, and T-cell protein tyrosine phosphatase. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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