Thermo Fisher Scientific VEGF Receptor 2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

Immunohistochemistry (IHC)

5-15 µg/mL

-

Flow Cytometry (Flow)

3-10 µg/mL

-

Neutralization (Neu)

0.1-0.3 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant extracellular domain of mouse VEGF receptor 2, expressed in mouse NSO cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_561911

Product Specific Information

Neutralization: Anti-Mouse VEGF Receptor 2 has the ability to neutralize the biological activity of recombinant mouse VEGF Receptor 2 on HUVE (human umbilical vein endothelial) cells in the presence of recombinant mouse VEGF Receptor 2/Fc (50 ng/mL) and recombinant mouse VEGF (5 ng/mL).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.