Thermo Fisher Scientific EphB4 Polyclonal Antibody
EphB4 단백질을 인식하는 Thermo Fisher Scientific의 염소 유래 폴리클로날 항체입니다. Western blot, IHC, Flow cytometry 등 다양한 응용에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| Immunohistochemistry (IHC) | 5–15 µg/mL |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | 0.5 µg/mL |
| Dot Blot (DB) | Assay-dependent |
| Inhibition Assays (IA) | 0.5–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified, recombinant mouse EphB4 extracellular domain, expressed in mouse NSO cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2100101 |
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
EPHB4 is a receptor tyrosine kinase belonging to the ephrin receptor family. Eph family kinases play critical roles in biological processes including nervous system development, angiogenesis, and synapse formation. Ephrin ligands are divided into ephrin-A (EFNA) and ephrin-B (EFNB) classes, with Eph receptors grouped based on extracellular domain sequence similarity and ligand binding specificity. EphB4 binds to ephrin-B2 and is essential for vascular development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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