Thermo Fisher Scientific Human CDK16 (PCTK1)/cyclin Y, GST Tag Recombinant Protein

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Baculovirus

Amino acid sequence

108-end; 2-end

Tag

GST-tag

Molecular weight

71.1-65.5 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 150mM NaCl, 0.1mM EDTA, 0.1mM PMSF, 0.25mM DTT, 10mM glutathione, 25% glycerol

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

Recombinant human CDK16 (107-end) and Cyclin Y (2-end) were co-expressed by baculovirus in Sf9 insect cells using N-terminal GST tags.

• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen™ Eu Kinase Binding Assay

Protein Form: Recombinant, Fragment

The gene accession number for the two subunits (CDK16[PCTK1] and Cyclin y) are NP_006192.1 and NP_659449.3.

Target Information

CDK16 (PCTK1)/cyclin Y belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. CDK16 plays a role in signal transduction cascades in terminally differentiated cells, in exocytosis, and in transport of secretory cargo from the endoplasmic reticulum. CDK16 is ubiquitously expressed with the highest levels detected in the brain and testis. CDK16 also contained the gene coding for ubiquitin-activating enzyme UBE1 that previously shown to mapped to Xp11.3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.