Thermo Fisher Scientific Noggin Monoclonal Antibody (OTI4C12), TrueMAB
Noggin 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC/IF에 사용 가능. Human 및 Dog 시료에 반응하며, 동결건조 형태로 제공. 재구성 후 1 mg/mL 농도로 사용 가능하며, 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4C12 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 28–232 of human noggin produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Noggin plays a critical role in numerous developmental processes such as neural tube fusion and joint formation. Organ morphogenesis is initiated by downgrowth from epithelial stem cells, mediated by signals from WNT3A (stabilizing β-catenin) and Noggin, which inhibits bone morphogenetic proteins.
Mutations in Noggin are associated with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1), both characterized by joint fusion defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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