Thermo Fisher Scientific Cyclin E Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Store vial at −20 °C prior to opening.
• Aliquot contents and freeze at −20 °C or below for extended storage.
• Avoid repeated freeze/thaw cycles.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4 °C as an undiluted liquid.
• Dilute only prior to immediate use.
• No reaction observed against other related cyclins.
• Cross-reactivity with Cyclin E from other species may occur.

Target Information

Cyclin E is a member of the highly conserved cyclin family, characterized by periodic changes in protein abundance through the cell cycle. Cyclins regulate CDK kinases, and each exhibits distinct expression and degradation patterns to coordinate mitotic events.
Cyclin E forms a complex with CDK2, essential for the G1/S transition. It accumulates at the G1–S boundary and is degraded during S phase. Overexpression has been linked to chromosomal instability and tumorigenesis.
Cyclin E interacts with and phosphorylates NPAT, a nuclear protein involved in histone gene expression and cell-cycle progression. Two alternatively spliced transcript variants produce distinct isoforms; additional variants have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.