Thermo Fisher Scientific APEX2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

ChIP assay (ChIP)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Suggested positive control: HeLa whole cell extract.

Target Information

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents, or by DNA glycosylases that remove specific abnormal bases.
AP sites are pre-mutagenic lesions that can prevent normal DNA replication, so the cell contains systems to identify and repair such sites.
Class II AP endonucleases cleave the phosphodiester backbone 5′ to the AP site.
This gene encodes a protein shown to have weak class II AP endonuclease activity.
Most of the encoded protein is located in the nucleus, but some is also present in mitochondria.
This protein may play an important role in both nuclear and mitochondrial base excision repair (BER).

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.