Thermo Fisher Scientific Phospho-SMAD2 (Ser250) Recombinant Rabbit Monoclonal Antibody (SD207-1)
Phospho-SMAD2 (Ser250) 인산화 부위를 인식하는 재조합 토끼 단클론 항체로, Western blot 및 유세포분석에 적합합니다. 높은 특이성과 재현성을 제공하며, 로트 간 일관성이 우수합니다. TGF-β 신호전달 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5000 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SD207-1 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser250 of Human Smad2 (aa 221–270) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809640 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal clones for production.
Key advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the large rabbit immune repertoire
Target Information
SMAD2 (MADH2, MAD2) regulates key cellular processes such as proliferation, apoptosis, and differentiation. It interacts with TGF-beta receptors through SMAD anchor for receptor activation (SARA).
Upon TGF-beta signaling, SMAD2 is phosphorylated, dissociates from SARA, and forms a complex with SMAD4 for nuclear translocation. This complex binds to target promoters and functions as a transcriptional regulator.
SMAD2 activity is modulated by ERK1-mediated phosphorylation and calmodulin interaction. It can also mediate activin signaling via activin type 1 receptor kinase.
Alternative splicing variants encoding SMAD2 have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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