Thermo Fisher Scientific CD95/FAS/TNFRSF6 Monoclonal Antibody (FAS, 3588)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Antibody is tested in direct ELISA.
• For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that acts as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. It also exists in a soluble form (~26 kDa).
Upon stimulation, FAS aggregates its intracellular FADD domains, forming the death-inducing signaling complex (DISC) and activating caspases. This cascade leads to apoptosis.
FAS also activates NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, contributing to proliferative signaling in fibroblasts and T cells.
Multiple alternatively spliced isoforms exist, with some lacking the transmembrane domain, which may negatively regulate apoptosis.
The Fas/Fas ligand system plays roles in diseases such as AIDS, hepatitis, and cancer, and is important in antiviral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.