Thermo Fisher Scientific CD95/FAS/TNFRSF6 Monoclonal Antibody (FAS, 3112)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Antibody is tested in direct ELISA.
• For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein acting as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. It also exists in a soluble form (~26 kDa).
Stimulation of FAS leads to aggregation of FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC), and activation of caspases.
Caspase activation triggers apoptosis via downstream signaling cascades.
FAS can also activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is involved in proliferative signaling in fibroblasts and T cells.
Multiple alternatively spliced variants exist, with some lacking the transmembrane domain, potentially regulating apoptosis negatively.
The Fas/Fas ligand system is implicated in diseases such as AIDS, hepatitis, and cancer, and is key in antiviral immune responses.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.