Thermo Fisher Scientific Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), NovaFluor Yellow 700, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.8 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• Request custom conjugation

Product Specific Information

The eBio13G3-19D monoclonal antibody reacts with mouse Ly108, the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6). Mouse Ly108 (also known as NTB-A) is expressed on NK, T, and B cells. Engagement of the Ly108 receptor enhances NK cell activity and IFN-gamma expression, and contributes to the inability of NK cells to kill Epstein-Barr virus-infected B cells in X-linked lymphoproliferative disease. CD4+ T cells from Ly108 knockout mice show reduced IL-4 production and defective neutrophil function.

Staining shows no strain differences between C57BL/6 and BALB/c mice; other strains not tested.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: For use in flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes and thymocytes at ≤0.8 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Antibody titration recommended for optimal performance.

NovaFluor Dye Compatibility

NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability with NovaFluor dyes.

CellBlox Plus Blocking Buffer

This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), which improves staining performance.
Use 5 µL per sample (regardless of the number of NovaFluors) when preparing antibody cocktails.
For single-color controls: add 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor Technology

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures designed to minimize spillover and spread.
Excitation: 552 nm; Emission: 700 nm; Laser: 561 nm (Yellow).

Note: Internal testing shows NovaFluor Yellow 700 may non-specifically stain B cells in SJL mice. No non-specific staining observed in BALB/c or C57BL/6 strains.

Target Information

Ly108 is the mouse homologue of human SLAMF6. It is expressed on NK, B, and T cells. Activation enhances NK cell activity and IFN-gamma expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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