Thermo Fisher Scientific Syrian Hamster IgG Isotype Control, Brilliant Violet 421, eBioscience

Applications

Tested Dilution

Flow Cytometry (Flow)

Assay-dependent

Control (Ctrl)

Assay-dependent

Product Specifications

Host/Isotype

Syrian hamster / IgG

Class

Polyclonal

Type

Isotype Control

Conjugate

Brilliant Violet™ 421 Brilliant Violet™ 421 Brilliant Violet™ 421

Excitation/Emission Max

406/423 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_3090512

Product Specific Information

Description: This hamster IgG fraction was purified by affinity chromatography from normal Golden Syrian Hamster serum.

Applications Reported: This antibody has been reported for use in flow cytometric analysis.

Applications Tested: This isotype control antibody has been tested by flow cytometric analysis of mouse splenocytes. It should be used at the same concentration as the experimental antibody.

Brilliant Violet™ 421 (BV421) is a dye that emits at 423 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.

Excitation: 407 nm; Emission: 423 nm; Laser: Violet Laser.

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.