Thermo Fisher Scientific Syrian Hamster IgG Isotype Control, Brilliant Violet 421, eBioscience
시리아 햄스터 IgG 아이소타입 컨트롤 항체로, Brilliant Violet 421 형광물질이 결합된 제품입니다. Flow Cytometry용 음성 대조로 사용되며, 405 nm 레이저 시스템에 적합합니다. 비특이적 결합 최소화를 위해 Brilliant Stain Buffer 사용 권장.
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Applications
Tested Dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (Flow) | Assay-dependent |
| Control (Ctrl) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Syrian hamster / IgG |
| Class | Polyclonal |
| Type | Isotype Control |
| Conjugate | Brilliant Violet™ 421 |
| Excitation/Emission Max | 406 / 423 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping conditions | Wet ice |
| RRID | AB_3090512 |
Product Specific Information
This hamster IgG fraction was purified by affinity chromatography from normal Golden Syrian Hamster serum.
This antibody has been reported for use in flow cytometric analysis.
This isotype control antibody has been tested by flow cytometric analysis of mouse splenocytes and should be used at the same concentration as the experimental antibody.
Brilliant Violet™ 421 (BV421) emits at 423 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please ensure your instrument can detect this fluorochrome.
When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.
Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
Isotype controls help differentiate non-specific background signal from specific antibody signal by providing a negative control without relevant antigen specificity.
They are commonly used in flow cytometry, but also applicable to ChIP, immunohistochemistry, and gel shift assays.
Isotype controls should match the primary antibody species and isotype for accurate comparison, and when using directly labeled antibodies, the control should have the same conjugate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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