Thermo Fisher Scientific Occludin Recombinant Mouse Monoclonal Antibody (OC-3F10)
Occludin 단백질을 인식하는 마우스 유래 재조합 단일클론 항체입니다. Western blot, ICC/IF, Flow Cytometry에 적합하며 높은 특이성과 재현성을 제공합니다. Expi293 시스템에서 발현되었으며, PBS 완충액에 보관됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:1,000–1:8,000 |
| Flow Cytometry (Flow) | 0.25 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | OC-3F10 |
| Immunogen | GST fusion protein consisting of the C-terminal region (~150aa) of human occludin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074175 |
Additional Formats
Product Specific Information
Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. These antibodies are generated from specific genes encoding the desired antibody, cloned into an expression vector, and expressed in vitro. Recombinant antibodies provide improved specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Occludin is a 65 kDa protein that can exist in multiple phosphorylated forms (up to ~82 kDa). It plays a role in regulating the localization and function of tight junctions. Polyunsaturated fatty acids can up-regulate occludin expression, enhancing transendothelial resistance and reducing permeability. Expression levels vary by tissue; high and continuous in brain tissue, lower and discontinuous in non-neural tissues. Occludin structure is conserved across species. Under-expression of occludin and other tight junction proteins contributes to increased permeability in tumor endothelial junctions, leading to brain tumor edema.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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