Thermo Fisher Scientific Vimentin Monoclonal Antibody (VI-RE/1)
휴먼 비멘틴 단백질을 인식하는 마우스 단클론 항체로 다양한 메센키말 세포 연구에 사용. WB, IHC, ICC, Flow, ELISA 등 다중 응용 가능. Protein A 정제, 1 mg/mL 농도, PBS buffer에 15mM sodium azide 포함. 4°C 보관, 동결 금지.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 5–10 µg/mL | - |
| Flow Cytometry (Flow) | 1–4 µg/mL | - |
| ELISA | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VI-RE/1 |
| Immunogen | Bacterially expressed full-length human vimentin (1–466 aa, UniProt ID: P08670-1, NCBI Node ID: 9606) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1088255 |
Additional Formats
Product Specific Information
This antibody reacts with human vimentin, a 57 kDa intermediate filament intracellular protein expressed in a wide variety of mesenchymal and mesodermal cell types.
This antibody does not cross-react with porcine or mouse proteins.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in the intracellular transport of proteins between the nucleus and plasma membrane.
During early cellular development, vimentin is co-expressed with desmin and later replaced by tissue-specific IFPs.
Vimentin also plays a role in steroid synthesis by facilitating cholesterol transport within mitochondria through cytoskeletal reorganization triggered by phosphorylation events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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