Thermo Fisher Scientific Fyn Monoclonal Antibody (FYN-01)
Fyn 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, IHC, ICC, IP 등 다양한 응용에 사용 가능. Human 및 Mouse 반응성. Protein A 정제, PBS buffer(15 mM sodium azide 포함) 보존. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Hamster, Human, Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FYN-01 |
| Immunogen | Recombinant fragment of human Fyn (aa 7–176) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1074491 |
Product Specific Information
This antibody recognizes Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase (intracellular antigen).
Immunoprecipitation example:
Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer containing N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8.2, 1% laurylmaltoside (w/v), 50 mM NaF).
The antibody FYN-01 provides a specific signal at 59 kDa and efficiently immunoprecipitates Fyn from cell lysates.
Target Information
FYN is a SRC-related kinase expressed in brain, skeletal muscle, and adipose tissues.
It functions in multiple signaling pathways and is involved in memory processes.
FYN encodes a membrane-associated tyrosine kinase that regulates cell growth and interacts with the p85 subunit of phosphatidylinositol 3-kinase and the fyn-binding protein.
Alternatively spliced transcript variants encoding distinct isoforms exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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