Thermo Fisher Scientific PGP9.5 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:5,000

-

Immunohistochemistry (IHC)

-

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 2 publications 2 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:500-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Horse, Human, Mouse, Pig, Rat

Published species

Cynomolgus monkey, Horse, Human, Mouse, Rat, Rhesus monkey

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant full length human UCHL1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PGP9.5,uniProtId:P09936-1,ncbiNodeId:9606,antigenRange:1-223,antigenLength:223,antigenImageFileName:PA1-10011_PGP9.5_P09936-1_Chicken.svg,antigenImageFileNamePDP:PA1-10011_PGP9.5_P09936-1_Chicken_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1088162

Product Specific Information

PA1-10011 was made against full length recombinant human UCHL1 expressed in and purified from E. coli and can be used to identify neurons and their processes in culture or in sections. The antibody works cleanly on appropriate lysates of cell and tissues. |Stable at 4°C.

Target Information

PGP9.5 (Protein gene product 9.5, UCH-L1, PARK5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. PGP9.5 has a molecular weight of 27 kDa and was first identified by high resolution two dimensional PAGE. PGP9.5 is a member of ubiquitin carboxyl-terminal hydrolase family 1 (peptidase family C12) with a ubiquitin carboxyl-terminal hydrolase domain. PGP9.5 is well known for having ubiquitin hydrolase and ligase activities that hydrolyzes small C-terminal adducts of ubiquitin to generate ubiquitin monomers. PGP9.5 is present in neurons and nerve fibers at all levels of the central and peripheral nervous system, in neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. Over expression of PGP9.5 leads to non-small cell lung cancer while decreased expression leads to Huntington disease and Alzheimer disease. Since PGP9.5 is present in cellular inclusions, it can be a useful as a neuronal marker and in the studies of neurodegenerative disorders such as with Parkinson disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.