Thermo Fisher Scientific Ubiquitin Polyclonal Antibody
Ubiquitin 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF, Flow Cytometry 등 다양한 응용 가능. 모든 종에 반응하며, 고순도 항원으로 제작. 연구용으로 단백질 분해 경로 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 | View 1 publication |
| Immunohistochemistry (IHC) | 1:1,000–1:2,000 | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:1,000–1:2,000 | - |
| Flow Cytometry (Flow) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | All, Human, Mouse, Rat |
| Published Species | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified ubiquitin conjugated with glutaraldehyde to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | 5 mM sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_1088148 |
Product Specific Information
PA1-10023 was made against purified bovine blood-derived ubiquitin coupled to keyhole limpet hemocyanin (KLH) with glutaraldehyde.
Target Information
Ubiquitin is a highly conserved protein involved in targeting cellular proteins for degradation by the 26S proteasome. It is synthesized as a precursor consisting of polyubiquitin chains or a single ubiquitin fused to another protein.
This gene encodes a fusion protein with ubiquitin at the N-terminus and ribosomal protein S27a at the C-terminus. Upon expression, it is processed to generate free ubiquitin and ribosomal protein S27a.
Ribosomal protein S27a is part of the 40S ribosomal subunit, contains C4-type zinc finger domains, and is located in the cytoplasm. Pseudogenes derived from this gene exist in the genome.
Similar fusion proteins include ribosomal proteins L40 and S30, which are synthesized with ubiquitin or ubiquitin-like proteins. Multiple alternatively spliced transcript variants encoding identical proteins have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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