Thermo Fisher Scientific RARB Monoclonal Antibody (R beta 28)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MA1-811 detects retinoic acid receptor (RAR) beta from human and mouse tissues.
This antibody shows slight cross-reactivity with RAR alpha when expressed in COS cells, but the signal is very low compared to RAR beta. It does not detect RAR alpha or gamma from cell lines or tissue extracts.

• Detects an ~52 kDa RAR beta protein in rat tissue extract by Western blot.
• Also detects an ~120 kDa unidentified protein, which can be competed away with the immunizing peptide.
• This higher molecular weight protein is notable in human SH-SY5Y and COS cells.
• Due to low native RAR expression, sensitive detection systems such as enhanced chemiluminescence (ECL) are recommended.

Reconstitution: Reconstitute with PBS.
Blocking Peptide: MA1-811 can be used with blocking peptide PEP-005.

Target Information

Retinoic Acid (RA), the active metabolite of vitamin A, regulates the transition of proliferating precursor cells to postmitotic differentiated cells.
The Retinoid X Receptors (RXRs: alpha, beta, gamma) preferentially bind 9-cis-RA and regulate gene transcription by forming heterodimers with RA receptors.
RA signaling plays key roles in neuronal development, synaptic plasticity, and may have therapeutic potential in certain cancers such as cervical cancer.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.