Thermo Fisher Scientific CD8 Monoclonal Antibody (LT8)

Applications

Immunohistochemistry (Frozen) (IHC (F))

• Assay-dependent

Flow Cytometry (Flow)

• Tested Dilution: 1:50–1:100
• Publications: 1 publication available

Immunoprecipitation (IP)

• Assay-dependent

Product Specifications

Product Specific Information

For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Mouse anti-human CD8 antibody, clone LT8 recognizes the human CD8 cell surface glycoprotein expressed by a subset of peripheral blood T cells with cytotoxic/suppressor activity.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein belonging to the immunoglobulin family of receptors. It plays a crucial role in signal transduction, T cell differentiation, and activation.
CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8α and CD8β, functioning as a co-receptor with the T cell receptor (TCR) for MHC class I molecules.
CD8α chain is essential for MHC-I binding and is also found as homodimers on subsets of T cells, NK cells, monocytes, and dendritic cells.
Engagement of MHC-I/peptide complexes triggers recruitment of Lck kinase, leading to lymphokine production, motility, and cytotoxic T lymphocyte (CTL) activation. Activated CTLs are vital for pathogen and tumor cell clearance.
Differentiation of naive CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 없음)