Thermo Fisher Scientific Phospho-c-Fos (Ser32) Polyclonal Antibody
Human, Mouse, Rat에서 반응하는 phospho-c-Fos (Ser32) 특이적 rabbit polyclonal antibody. Western blot과 IHC(Paraffin)에 최적화된 고감도 검출용. 액상 형태, 1 mg/mL 농도, -20°C 보관. c-Fos의 Ser32 인산화 상태만 선택적으로 인식.
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Thermo Fisher Scientific Phospho-c-Fos (Ser32) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human FOS (Accession P01100), corresponding to amino acid residues around phosphorylated Ser32 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816032 |
Product Specific Information
Antibody detects endogenous levels of c-Fos only when phosphorylated at Ser32.
Target Information
FBJ murine osteosarcoma viral oncogene homolog, commonly known as Cellular protooncogene Fos (c-Fos), belongs to the fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14.
c-Fos heterodimerizes with c-Jun to form the Activator Protein-1 (AP-1) transcription factor, which regulates transcription of several genes involved in cellular signal transduction, proliferation, and differentiation.
c-Fos is phosphorylated by various stimuli, including growth factors and insulin, at multiple sites. The best-known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK, which controls nuclear localization and transcriptional activity of c-Fos.
Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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