Thermo Fisher Scientific Acetyl-FOXO1 (Lys294) Polyclonal Antibody
FOXO1 단백질의 Lys294 아세틸화 상태를 특이적으로 검출하는 Rabbit Polyclonal 항체. Western blot, IHC, IP에 적합하며, 인간, 마우스, 랫드 등 다양한 종에 반응. 액상 형태로 -20°C 보관, 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunoprecipitation (IP) | – | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human FOXO1 (Accession Q12778), corresponding to amino acid residues E282–H302. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816035 |
Product Specific Information
This antibody detects endogenous levels of Acetyl-FOXO1A only when acetylated at Lys294.
Target Information
FOXO1 (FKHR, Forkhead Box O1) is a transcription factor belonging to the forkhead family. It can function as a coactivator or corepressor of nuclear receptor activity through its LXXLL motif. FOXO1 is associated with PAX3 in alveolar rhabdomyosarcoma and plays key roles in autophagy, myogenic differentiation, and tumor suppression. It regulates metabolic homeostasis in response to oxidative stress and binds to the insulin response element (IRE) and Daf-16 family binding element (DBE). FOXO1 influences redox balance, osteoblast activity, and bone mass, and modulates glucose metabolism by controlling genes such as IGFBP1, G6PC, and PCK1. It acts downstream of CDK1, PKB/AKT1, and MST1 in regulating cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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