Thermo Fisher Scientific MEP1B Polyclonal Antibody
Thermo Fisher Scientific의 MEP1B Polyclonal Antibody는 Mouse Meprin beta Subunit을 인식하는 Goat IgG 항체입니다. Western blot, IHC(F), IP 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1 µg/mL | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 5–15 µg/mL | - |
| Immunoprecipitation (IP) | 25 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse Meprin beta Subunit/MEP1B (Leu21–Ser594; Thr75Ile, Ile432Val) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2607364 |
Product Specific Information
- In Western blots, approximately 40% cross-reactivity with recombinant human MEP1B is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Meprins are multidomain zinc metalloproteases highly expressed in mammalian kidney and intestinal brush border membranes, leukocytes, and certain cancer cells. They hydrolyze various peptide and protein substrates and are implicated in cancer and intestinal inflammation.
Mature meprins form oligomers of alpha and/or beta subunits: alpha homooligomers are secreted, while oligomers containing the beta subunit are plasma membrane-bound.
The MEP1B gene encodes the beta subunit. Its disruption in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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