Thermo Fisher Scientific PAG1 Monoclonal Antibody (PAG-C1)
PAG1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, IHC, IP 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 등에서 반응하며, 단백질 A로 정제된 액상 형태입니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- MA119287
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific PAG1 Monoclonal Antibody (PAG-C1)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Published Species | Human, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PAG-C1 |
| Immunogen | C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2160061 |
Product Specific Information
This antibody recognizes an epitope located in the intracellular C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which migrates on SDS-PAGE gels anomalously as an 80 kDa molecule.
- Positive control for IP: RAJI human Burkitt lymphoma cell line
- Positive control for IHC: Appendix (germinal center of lymphatic follicle)
- IHC recommendation: Heat-mediated antigen retrieval in citrate buffer, pH 6.1
Target Information
PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts. It migrates on SDS-PAGE gels as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases.
Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to increased PAG tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding, and increased activation of the protein tyrosine kinase Lck.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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