Thermo Fisher Scientific PAG1 Monoclonal Antibody (PAG-C1)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody recognizes an epitope located in the intracellular C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which migrates on SDS-PAGE gels anomalously as an 80 kDa molecule.

• Positive control for IP: RAJI human Burkitt lymphoma cell line
• Positive control for IHC: Appendix (germinal center of lymphatic follicle)
• IHC recommendation: Heat-mediated antigen retrieval in citrate buffer, pH 6.1

Target Information

PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts. It migrates on SDS-PAGE gels as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases.
Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to increased PAG tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding, and increased activation of the protein tyrosine kinase Lck.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.