Thermo Fisher Scientific EBP50 Monoclonal Antibody (EBP-10)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein.
For use in Western blot applications, MA1-19291 should be placed in a vertical incubator for 60 minutes.
A suggested positive control for this product is RAJI human lymphoma cell lysate.
Western Blot: Reducing conditions.

Target Information

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50), is an adaptor protein that associates with beta-catenin and is required for its localization at cell-cell junctions.
It interacts with various G protein-coupled receptors and regulates their trafficking, sodium-hydrogen exchange, and sodium-dependent phosphate transport.
NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth.
It contains a C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains, enabling a head-to-tail intramolecular conformation to regulate its interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.