Thermo Fisher Scientific EBP50 Monoclonal Antibody (EBP-10)
EBP50 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, IP 등에 사용 가능. NHERF1/EBP50 인산화 단백질 검출에 적합하며, Protein A로 정제된 액상 항체. 4°C에서 보관, 연구용 전용.
- 카탈로그번호
- MA119291
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | EBP-10 |
| Immunogen | Bacterially produced, recombinant, full-length human NHERF1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1074133 |
Product Specific Information
This antibody reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein.
For use in Western blot applications, MA1-19291 should be placed in a vertical incubator for 60 minutes.
A suggested positive control for this product is RAJI human lymphoma cell lysate.
Western Blot: Reducing conditions.
Target Information
NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50), is an adaptor protein that associates with beta-catenin and is required for its localization at cell-cell junctions.
It interacts with various G protein-coupled receptors and regulates their trafficking, sodium-hydrogen exchange, and sodium-dependent phosphate transport.
NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth.
It contains a C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains, enabling a head-to-tail intramolecular conformation to regulate its interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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