Thermo Fisher Scientific EpCAM Monoclonal Antibody (323/A3)
EpCAM(CD326)을 인식하는 Mouse Monoclonal Antibody(323/A3)로, Human 시료에 반응합니다. WB, IHC, ICC, Flow, IP 등 다양한 응용에 사용 가능하며, 비결합(Unconjugated) 형태로 제공됩니다. Protein A 정제, 4°C 보관, 연구용 전용 제품입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–4 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | 1–10 µg/mL | – |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL | View 2 publications |
| Flow Cytometry (Flow) | 1–4 µg/mL | View 2 publications |
| Immunoprecipitation (IP) | 1–4 µg/mL | – |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 323/A3 |
| Immunogen | Human breast cancer MCF-7 cells (EpCAM/CD326, UniProt ID: P16422-1, aa 1–314) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154429 |
Available Conjugated Formats
Product Specific Information
This antibody recognizes an extracellular epitope of CD326/EpCAM, a marker of epithelial lineages that is overexpressed in many carcinomas.
It does not cross-react with rat.
Western Blot: use under non-reducing conditions.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent homotypic cell adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cyclins A and E.
Ep-CAM expression negatively regulates cadherin-mediated adhesions, affecting epithelial differentiation and growth.
Overexpression correlates with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial-origin tumors.
Ep-CAM is typically localized to the basolateral surface of simple epithelia and many carcinoma types, and can help distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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