Thermo Fisher Scientific EpCAM Monoclonal Antibody (VU-1D9)
EpCAM 단백질을 인식하는 VU-1D9 클론의 마우스 단일클론 항체로, 다양한 인간 상피세포 및 암조직에서 강한 염색을 보임. WB, IHC, ICC, Flow 등 다양한 응용 가능. 비결합형 액상 형태로 제공되며, 4°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–4 µg/mL | View 3 publications |
| Immunohistochemistry (IHC) | – | View 6 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–10 µg/mL | – |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | – |
| Immunocytochemistry (ICC/IF) | 4 µg/mL | View 2 publications |
| Flow Cytometry (Flow) | 1–3 µg/mL | View 1 publication |
| Immunoprecipitation (IP) | 1–2 µg/mL | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VU-1D9 |
| Immunogen | Small cell lung carcinoma cell line H69 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153547 |
Available Conjugated Formats:
Product Specific Information
This antibody recognizes an extracellular epitope within the EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. It strongly stains various normal epithelial cells and carcinomas.
Immunohistochemistry (Paraffin):
Antigen retrieval: heat (sodium citrate) + trypsin.
Target Information
EpCAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent cell adhesion molecule and influences cell cycle, proliferation, and metabolism through induction of proto-oncogene c-myc and cyclins A and E.
EpCAM-mediated adhesions negatively regulate cadherin-mediated adhesions, impacting epithelial differentiation and growth. Overexpression of EpCAM is associated with enhanced epithelial proliferation and is a marker for tumors of epithelial lineage. It is expressed on the basolateral surface of most simple epithelia and many carcinoma types and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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