Thermo Scientific™

DyLight™ 650 Maleimide

Thermo Scientific DyLight 650 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 650 used to fluorescently label sulfhydryl-containing peptides,자세히 알아보기

Thermo Scientific DyLight 650 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 650 used to fluorescently label sulfhydryl-containing peptides, proteins, and other biomolecular probes.

DyLight 650 provides vibrant far-red fluorescence with comparable or improved performance over other dyes, including Alexa Fluor™ 647 and Cy5™ dye, over a broad pH range (pH 4-9). The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.

Features of DyLight 650 Maleimide:

• High performance—DyLight 650 fluoresces brighter than Alexa Fluor 647 and Cy5
• Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
• Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
• Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiency

Applications:
• Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
• Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies

DyLight 650 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5–7.5. Learn more about maleimide chemistry.

사양

설명DyLight 650 Maleimide

수량1 mg

제품 유형Dye

용해도DMF (Dimethylformamide)

화학물질 반응성Thiol

반응성 부분Maleimide

라벨 또는 염료DyLight™ 650

라벨 유형DyLight Fluors

제품라인DyLight™

방출672

여기652

Unit SizeEach