Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 350
DyLight 350 형광으로 결합된 염소 유래 항-마우스 IgG (H+L) 2차 항체로, WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합합니다. 높은 특이성과 낮은 비특이적 결합을 제공하며, 신뢰성 있는 형광 검출에 이상적입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:2,000 |
| Flow Cytometry (Flow) | 1:25–1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Mouse IgG, whole molecule |
| Conjugate | DyLight™ 350 |
| Excitation / Emission Max | 353 / 432 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C (protected from light) |
| Shipping Conditions | Ambient |
| RRID | AB_1965940 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Product #62271 has been successfully used in Western blot, IF, ICC, IHC, IP, and FACS applications.
This antibody reacts with the heavy chains of mouse IgG and light chains common to most mouse immunoglobulins, but does not react with non-immunoglobulin serum proteins.
Cross-reactivity with immunoglobulins from other species may occur.
Store protected from light at 4°C.
For extended storage, add an equal volume of glycerol (final ~50%) and store at -20°C.
DyLight 350 Amax = 353 nm; Emax = 432 nm.
Mole Dye/Mole Protein Ratio is lot-dependent.
Target Information
Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins.
They are useful for detection, sorting, or purification of target proteins and offer high sensitivity through signal amplification.
Typically generated by immunizing the host with pooled immunoglobulins, these antibodies can be further purified or conjugated (e.g., HRP, AP, fluorescence) for specific applications.
Available Conjugate Formats
- Agarose
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 647
- Alexa Fluor 680
- Alexa Fluor 790
- Alexa Fluor Plus 405 / 488 / 647
- AP, bGal, Biotin
- DyLight 405 / 488 / 550 / 594 / 633 / 650 / 680 / 800 4X PEG
- FITC, HRP, PE, Rhodamine, TRITC
- WesternDot 625
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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