
Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 350
DyLight 350 형광 표지를 가진 Goat anti-Rabbit IgG (H+L) 2차 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 면역실험에 적합합니다. 고순도 항원 친화 크로마토그래피로 정제되었으며, 높은 특이성과 민감도를 제공합니다.
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Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 350
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:2,000 |
| Flow Cytometry (Flow) | 1:25–1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Rabbit IgG, whole molecule |
| Conjugate | DyLight™ 350 |
| Excitation/Emission Max | 353 / 432 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient |
| RRID | AB_1965941 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 488-10 nm colloidal gold
- Alexa Fluor 488-5 nm colloidal gold
- AP
- Biotin
- DyLight 405
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 633
- DyLight 650
- DyLight 680
- DyLight 800 4X PEG
- FITC
- HRP
- PE-Cyanine5.5
- TRITC
- WesternDot 625
- Custom conjugation available
Product Specific Information
Product #62270 has been successfully used in Western blot, IF, ICC, IHC, IP, and FACS applications.
This antibody reacts with the heavy chains of rabbit IgG and the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. Cross-reactivity with immunoglobulins from other species may occur.
Store protected from light at 4°C. To extend shelf life, add an equal volume of glycerol (final 50%) and store at -20°C.
DyLight 350 Amax = 353 nm; Emax = 432 nm. Mole Dye/Mole Protein Ratio is lot-dependent.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified reagents specific for rabbit immunoglobulins. They are used for detection, sorting, or purification of rabbit IgG targets.
Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondaries can bind to a single primary antibody.
These antibodies are typically generated by immunizing host animals with pooled immunoglobulins from the target species, followed by purification and conjugation for high specificity.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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