Thermo Fisher Scientific Phospho-LC3B (Thr29) Polyclonal Antibody
LC3B 단백질의 Thr29 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot 및 IHC(P) 검증 완료. 자가포식 관련 연구용으로 적합하며, 고순도 정제 및 안정적 PBS/glycerol 저장용액 제공.
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Thermo Fisher Scientific Phospho-LC3B (Thr29) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MAP1LC3B (Accession Q9GZQ8), corresponding to amino acid residues around phosphorylated Thr29. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816574 |
Product Specific Information
Antibody detects endogenous levels of LC3B only when phosphorylated at Thr29.
Target Information
LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) in humans is encoded by the gene MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B).
LC3B is associated with microtubule assembly and plays an important role in neurogenesis.
Recent studies indicate that LC3B is crucial in autophagy, the process responsible for bulk degradation of cytoplasmic components.
Three human LC3 isoforms undergo post-translational modifications during autophagy. Macroautophagy is the major inducible pathway for turnover of cytoplasmic constituents and degradation of active cytoplasmic enzymes and organelles during nutrient starvation.
LC3B mediates interactions between microtubules and cytoskeletal components and may be involved in cancer, aging, metabolic and neurodegenerative disorders, as well as cardiovascular and pulmonary diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: PA5-105101_LC3B_Q9GZQ8-1_Rabbit.svg, PA5-105101_LC3B_Q9GZQ8-1_Rabbit_PDP.jpeg)
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