Thermo Fisher Scientific Mouse IgG2b kappa Isotype Control (eBMG2b), Super Bright 702, eBioscience
Flow cytometry용 Mouse IgG2b kappa 동형 대조 항체로 Super Bright 702 형광체 결합. Violet 레이저(405 nm)에서 여기되어 702 nm에서 방출. 실험 항체의 음성 대조로 사용 가능하며, 빛에 민감하므로 암조건에서 보관 필요.
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Thermo Fisher Scientific Mouse IgG2b kappa Isotype Control (eBMG2b), Super Bright 702, eBioscience
Applications and Tested Dilution
- Flow Cytometry (Flow): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBMG2b |
| Conjugate | Super Bright™ 702 |
| Excitation / Emission Max | 413 / 702 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2688281 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV series (395–805)
- Brilliant Violet series (421–786)
- eFluor 450, 506, 570, 615, 660
- FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright series (436, 600, 645, 780)
- Functional Grade
- Custom conjugation available
Product Specific Information
The monoclonal mouse IgG2b is useful as an isotype control immunoglobulin.
Applications Reported:
This Mouse IgG2b, Kappa Isotype Control PE-Cyanine7 has been reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells.
Use this isotype control at the same concentration as the experimental antibody.
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect the vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency.
Fluorophore Information:
Super Bright 702 is a tandem dye excited with the violet laser line (405 nm) and emits at 702 nm.
Recommended filter: 710/50 bandpass.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product #SB-4401) to minimize non-specific polymer interactions.
Laser: Violet (405 nm)
Emission: 702 nm
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Mouse IgG2b is a subtype of immunoglobulin G produced by B cells in response to antigens.
It is the third most abundant IgG subtype in serum and plays a role in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity.
It regulates immune responses, including T cell activation and B cell inhibition.
Widely used in research and diagnostic applications such as ELISA, Western blotting, and immunohistochemistry, as well as in therapeutic research like immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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