Thermo Fisher Scientific CD4 Monoclonal Antibody (YKIX302.9), Super Bright 702, eBioscience
Thermo Fisher Scientific의 CD4 Monoclonal Antibody (YKIX302.9), Super Bright 702은 개(Canine) CD4 항원을 표적하는 rat IgG2a 항체입니다. Flow Cytometry에 최적화되어 있으며, Super Bright 702 형광체로 탠덤 염색이 가능합니다. Violet laser(405 nm)로 여기되어 702 nm에서 방출됩니다. 광민감성 물질로 어둠 속 4°C에서 보관해야 ...
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Thermo Fisher Scientific CD4 Monoclonal Antibody (YKIX302.9), Super Bright 702, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution
- 5 µL (0.25 µg)/test
Publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 702, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | YKIX302.9 |
| Conjugate | Super Bright™ 702 |
| Excitation / Emission Max | 413 / 702 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744889 |
Additional Formats
Product Specific Information
The YKIX302.9 monoclonal antibody reacts with canine CD4, a cell surface receptor expressed on thymocytes, mature T cells, dendritic cells, and neutrophils. CD4 binds to MHC class II on antigen-presenting cells, playing a key role in T cell development and homeostasis.
This antibody blocks MHC II-restricted T cell responses in vivo and in vitro.
Reported for use in flow cytometric analysis and tested with normal canine peripheral blood cells at 5 µL (0.25 µg)/test. A test corresponds to staining a 100 µL cell sample (10⁵–10⁸ cells/test).
Super Bright 702 is a tandem dye excited by a 405 nm violet laser and emits at 702 nm. Recommended filter: 710/50 bandpass. Ensure instrument compatibility.
When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Light sensitivity: Protect vial and samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using:
- IC Fixation Buffer (Product # 00-8222)
- 1-step Fix/Lyse Solution (Product # 00-5333)
Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD4 antigen recognizes MHC class II molecules and serves as a co-receptor for HIV. Expressed mainly in T helper cells, but also in monocytes, macrophages, and dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and certain regions of brain and gut.
CD4 aids early T-cell activation via association with TCR complex and Lck kinase. It may mediate neuronal damage in infectious or immune-mediated CNS diseases. Multiple alternatively spliced transcripts are known (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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