Thermo Fisher Scientific Phospho-Cdc25B (Ser149) Polyclonal Antibody
Cdc25B 단백질의 Ser149 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, ELISA 등 다양한 응용 가능. Human 및 Mouse 반응성. 고순도 Protein A 정제, 안정적인 액상 형태로 -20°C 보관. 연구용 전용 제품.
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Thermo Fisher Scientific Phospho-Cdc25B (Ser149) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from mouse Cdc25B around the phosphorylation site of Ser149 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in an ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have different levels of activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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