Thermo Fisher Scientific Caspase 9 (Cleaved Asp330) Polyclonal Antibody
Caspase 9 단백질의 Asp330 절단 부위를 인식하는 토끼 폴리클로날 항체로, Western blot, IHC, ICC/IF에 적합합니다. 인간 시료에 반응하며, 액상 형태로 제공됩니다. 세포사멸 관련 단백질 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CASP9 (Accession P55211), corresponding to amino acid residues E313–S339 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | –20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816722 |
Product Specific Information
This antibody detects endogenous levels of the fragment of activated Caspase 9 resulting from cleavage adjacent to Asp330.
Target Information
Caspase 9 (also known as ICE-like apoptotic protease 6, LAP6, Mch6, Apaf3) is a member of the peptidase family C14 containing a CARD domain. Caspases are cysteine proteases expressed as inactive precursors that mediate apoptosis by proteolysis of specific substrates. Caspase 9 functions as a heterotetramer, localizes in the cytosol, and translocates to mitochondria upon activation. It plays a key role in the caspase activation cascade responsible for apoptosis execution, cleaving and activating Caspase 3 and Caspase 6. Caspase 9 also promotes DNA damage-induced apoptosis in an ABL1/c-Abl-dependent manner and cleaves poly(ADP-ribose) polymerase (PARP). It is expressed in various human tissues, and its dysfunction is associated with abnormal brain development, cancer, and neurodegenerative diseases such as Alzheimer’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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