Thermo Fisher Scientific ERp72 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:500

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Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues K(629) F I E E H A T K L S R T K E E L(645) of humna ERp72. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ERp72,uniProtId:P13667-1,ncbiNodeId:9606,antigenRange:630-645,antigenLength:645,antigenImageFileName:PA1-007_ERp72_P13667-1_Rabbit.svg,antigenImageFileNamePDP:PA1-007_ERp72_P13667-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2160971

Product Specific Information

PA1-007 detects ERp72 protein in human and mouse samples.

PA1-007 has successfully been used in ICC, IF and Western blot procedures. By Western blot, this antibody detects a 72 kDa protein representing ERp72 in human fibrosarcoma HT 1080 cell lysate.

The PA1-007 immunogen is a synthetic peptide corresponding to residues K(629) F I E E H A T K L S R T K E E L(645) of humna ERp72. This peptide (Cat. # PEP-223) is available for use in neutralization and control experiments.

Target Information

ERp72 is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. Sequence analysis of ERp72 shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.