Thermo Fisher Scientific Dynamin 1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 10–50 µg/mL
• Publications: View 2 publications

Immunohistochemistry (IHC)

• Tested Dilution: 10–50 µg/mL
• Publications: View 2 publications

Miscellaneous PubMed (Misc)

• Publications: View 2 publications

Product Specifications

Product Specific Information

• Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
• The peptide is homologous in human and mouse.
• Specificity: Dynamin 1.

Target Information

Dynamin is a GTPase required for endocytosis of clathrin-coated vesicles from the membrane. Its role in endocytosis was first discovered in the temperature-sensitive parylytic shibire mutation of Drosophila melanogaster.
Three closely related isoforms have been identified in mammals:

• Dynamin 1: Expressed exclusively in neurons
• Dynamin 2: Ubiquitously expressed
• Dynamin 3: Expressed primarily in testes

Each isoform has several splice variants, suggesting distinct intracellular targets or vesicle budding functions. Common domain features include:

• N-terminal GTPase domain
• Central pleckstrin homology domain
• Proline-rich C-terminal region with multiple SH3 domains

These SH3 domains target dynamin to clathrin-coated pits. Dynamin interacts with proteins such as the AP2 adaptor complex and amphiphysin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.