Thermo Fisher Scientific Dynamin 1 Polyclonal Antibody
Dynamin 1 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot 및 Immunohistochemistry에 사용 가능. 인간, 생쥐, 랫트 반응성. Lyophilized 형태로 보관 용이하며, 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:300–1:2,000 | - |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from the C-terminal region of rat Dynamin 1 conjugated to an immunogenic carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 150 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The antigen is homologous in human and mouse.
- Specificity: Dynamin 1.
Target Information
Dynamin is a GTPase required for endocytosis of clathrin-coated vesicles from the membrane. Its role was first discovered in Drosophila melanogaster through the temperature-sensitive shibire mutation.
Three isoforms exist in mammals: Dynamin1 (neurons), Dynamin2 (ubiquitous), and Dynamin3 (testes).
Each isoform has multiple splice variants, suggesting distinct intracellular functions.
Common domain features include:
- N-terminal GTPase domain
- Central pleckstrin homology domain
- Highly basic proline-rich C-terminal region with Src homology 3 (SH3) domains
These SH3 domains target dynamin to clathrin-coated pits. Dynamin interacts with AP2 adaptor protein complex and amphiphysin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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