Thermo Fisher Scientific EGFR Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 15 publications 15 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunocytochemistry (ICC/IF)

1 µg/mL

View 4 publications 4 publications

Immunoprecipitation (IP)

2 µg

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C L(1198) R V A P Q S S E F I G A(1210) of human EGFR. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EGFR,uniProtId:P00533-1,ncbiNodeId:9606,antigenRange:1198-1210,antigenLength:1210,antigenImageFileName:PA1-1110_EGFR_P00533-1_Rabbit.svg,antigenImageFileNamePDP:PA1-1110_EGFR_P00533-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_326079

Product Specific Information

PA1-1110 detects EGFR from human, non-human primate (monkey), mouse, and rat samples.

PA1-1110 has been successfully used in Western blot, immunoprecipitation, immunohistochemistry, and immunofluorescence procedures. By Western blot, this antibody detects ~190 kDa protein representing EGFR from human HeLa cell extract.

The PA1-1110 immunogen is a synthetic peptide corresponding to residues C L(1198) R V A P Q S S E F I G A(1210) of human EGFR.

Target Information

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.